1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of nitiomidazoles in the tissue, liver, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The nitiomidazoles in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density(OD) value of the sample has a negative correlation with nitiomidazoles concentration in the sample. This value is compared to the standard curve and the content of the corresponding nitiomidazoles is subsequently obtained.
3. Components
1）Microplate: 12 strips with 8 removable wells each
2）6× standard solution (1 mL each): 0 ppb, 1ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb
3）Enzyme conjugate (7mL).....................................red cap
4）Antibody solution (7 mL).....................................blue cap
5）Substrate A solution (7 mL)................................. brown cap
6）Substrate B solution (7 mL)..................................black cap
7）Stop solution (7 mL) ......................................... yellow cap
8）Washing solution (40 mL 20× )............................white cap
9）Redissolving solution (50 mL 2×).........................blue cap