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Tetrodotoxin ELISA Kit
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| Post Date: | Feb 19,2023 |
| Expiry Date: | Feb 19,2024 |
| Detailed Description: |
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tetrodotoxin in the tissue, liver, fish. The coupling antigens are pre-coated on the micro-well stripes. The tetrodotoxin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-TTX antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Tetrodotoxin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Tetrodotoxin is subsequently obtained. 2. Technical specifications Sensitivity : 8 ppb Detect time: Approx. 75 min Detection limit: Tissue, liver......................... 8 ppb Recovery rate: Tissue, liver........................90 ± 10% Cross-reaction rate: Tetrodotoxin...................... 100% 3. Components 1）Microplate: 12 strips with 8 removable wells each 2）6× Standard Solution (1 mL each): 0 ppb, 8 ppb, 16 ppb, 32 ppb, 64 ppb, 128 ppb 3）Antibody Solution /Enzyme Conjugated Antibody (5μL 10000× ) 4）Substrate Solution (12 mL) 5）Stop Solution (7 mL) 6）Washing Solution (30 mL 20× ) 7）PBS Solution ( 10 mL ) |
| Company: | Wuhan Ecalbio Co.,Ltd [ China ] |
| Contact: | Ku |
| Tel: | 027-87745856 |
| Fax: | - |
| Email: | info@ecalbio.com |
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