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Nitiomidazoles ELISA Kit
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| Post Date: | Feb 19,2023 |
| Expiry Date: | Feb 19,2024 |
| Detailed Description: |
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of nitiomidazoles in the tissue, liver, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The nitiomidazoles in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density(OD) value of the sample has a negative correlation with nitiomidazoles concentration in the sample. This value is compared to the standard curve and the content of the corresponding nitiomidazoles is subsequently obtained. 2. Technical specifications Sensitivity : 1 ppb Detect time: Approx. 75 min Detection limit: Tissue, liver, intestine,shrimp,fish,honey .......... 3 ppb Milk ................................................................... 40 ppb Recovery rate: Tissue, liver, honey, intestine,shrimp,fish,milk ....... 80 ±10% Milk ................................................................ 85 ±10% Cross-reaction rate: Metronidazole.................. 100 % Tinidazole ....................... 90 % Ornidazole ..................... 80 % Secnidazole...................... 65 % 3. Components 1）Microplate: 12 strips with 8 removable wells each 2）6× standard solution (1 mL each): 0 ppb, 1ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb 3）Enzyme conjugate (7mL).....................................red cap 4）Antibody solution (7 mL).....................................blue cap 5）Substrate A solution (7 mL)................................. brown cap 6）Substrate B solution (7 mL)..................................black cap 7）Stop solution (7 mL) ......................................... yellow cap 8）Washing solution (40 mL 20× )............................white cap 9）Redissolving solution (50 mL 2×).........................blue cap |
| Company: | Wuhan Ecalbio Co.,Ltd [ China ] |
| Contact: | Ku |
| Tel: | 027-87745856 |
| Fax: | - |
| Email: | info@ecalbio.com |
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