sequence recombinant Carboxypeptidase B,from E.coli

• Category :

  Pharmaceuticals and Biochemicals/Biochemicals and biotech products

• Synonyms :

  carboxypeptidase B from procine*pancreas protease;Carboxypeptidase B;Carboxypeptidase B, AEBSF treated hog pancreas;Recombinant Carboxypeptidase B,from E.coli;Sequencing recombinant Carboxypeptidase B,from E.coli;Carboxypeptidase B,recombinant from E.coli;Peptidy-L-lysine(L-arginine);protaminase;

• CAS NO :

  9025-24-5

• EC NO :

  232-788-9

• Molecular Formula :

• Molecular Weight :

• Main Specifications :

  mg;g;bulk

• Packing :

  Aluminum Foil Bag

• Product description :

  Sequencing Grade Carboxypeptidase B Animal Origin Free SOURCE Expressed in E. Coli, purified by HPLC. DESCRIPTION Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation. MAIN FEATURES Source E.Coli Purified by HPLC Physical form White or white-like lyophilized powder Specific activity NLT 200 units/mg pro. No Contaminant activity No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant. APPLICATION Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. Antibody quality control. RECOMMEND USAGE To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.5. The ratio to aimed protein is 1:500 to 1:1000 (w/w), the optimum pH is pH7-9. STORAGE AND STABILITY Recommend recombinant carboxypeptidase B lyophilized powder stored under 2-8℃ in sealed container. It is stable within 24 months. After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing. RELATED PRODUCT: Recombinant Trypsin (sequencing purity), Recombinant Carboxypeptidase B (sequencing purity), V8 (sequencing purity), Recombinant Chymotrypsin (sequencing purity)

• Uses :

  Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.