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Uses : Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.

Product description: Sequencing Grade V8 Protease FULL NAME Endoproteinase Glu-C,from Staphylococcus aureus strain V8,V8 Protease CAS NUMBER 66676-43-5 EC NUMBER 3.4.21.19 DESCRIPTION Staphylococcus aureus Protease V8 (Endoproteinase GluC) is a serine proteinase that selectively cleaves peptide bonds C-terminal to glutamic acid residues. It also cleaves at aspartic acid residues.The Optimum pH is 8.0 ~ 8.5. Its enzyme inhibitors are Phosphoric acid diisopropyl ester fluoride (DFP) and alpha 2 - macroglobulin and Na-p-tosyl-L-lysine chloromethyl ketone (TLCK). SPECIFIC ACTIITY approx. 20 U/mg at 25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approx. 500 U/mg at 37°C with casein as the substrate). INSTRUCTIONS FOR USE Cutting condition: pH 8.0~8.5 PACKAGING 1U/vial, 20 mg/vial STORAGE -20°C. APPLICATION Protein structure and sequence analysis RELATED PRODUCT: Recombinant Trypsin（sequencing purity）,Recombinant Carboxypeptidase B（sequencing purity）,V8（sequencing purity）, Recombinant Chymotrypsin（sequencing purity）