Tetrodotoxin ELISA Kit

• Post Date:

  Feb 19,2023

• Expiry Date:

  Feb 19,2024

• Detailed Description:

  1. Principle
  This test kit is based on the competitive enzyme immunoassay for the detection of Tetrodotoxin in the tissue, liver, fish. The coupling antigens are pre-coated on the micro-well stripes. The tetrodotoxin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-TTX antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Tetrodotoxin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Tetrodotoxin is subsequently obtained.
  
  2. Technical specifications
  Sensitivity : 8 ppb Detect time: Approx. 75 min
  
  Detection limit:
  Tissue, liver......................... 8 ppb
  
  Recovery rate:
  Tissue, liver........................90 ± 10%
  
  Cross-reaction rate:
  Tetrodotoxin...................... 100%
  
  3. Components
  1）Microplate: 12 strips with 8 removable wells each
  2）6× Standard Solution (1 mL each):
  0 ppb, 8 ppb, 16 ppb, 32 ppb, 64 ppb, 128 ppb
  3）Antibody Solution /Enzyme Conjugated Antibody (5μL 10000× )
  4）Substrate Solution (12 mL)
  5）Stop Solution (7 mL)
  6）Washing Solution (30 mL 20× )
  7）PBS Solution ( 10 mL )

• Company:

  Wuhan Ecalbio Co.,Ltd     [ China ]        

• Contact:

  Ku

• Tel:

  027-87745856

• Fax:

  -

• Email:

  info@ecalbio.com